Incubation:Article Title: Numb exon 9 inclusion regulates Integrinβ5 surface expression and promotes breast cancer metastasis.
Article Snippet: The endocytic adaptor protein Numb acts as a tumor suppressor through downregulation of oncogenic pathways in multiple cancer types.. The identification of splicing alterations giving rise to changes in Numb protein isoform expression indicate that Numb also has tumor promoting activity, though the underlying mechanisms are unknown.. Here we report that NUMB exon 9 inclusion, which results in production of a protein isoform with an additional 49 amino acids, is a feature of multiple cancer types including all subtypes of breast cancer and correlates with worse progression-free survival.
Biomarker Discovery:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039.
Immunostaining:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039.
Transgenic Assay:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039.
Chromatin Immunoprecipitation:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University).
Flow Cytometry:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University).
Magnetic Resonance Imaging:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University).
Immunofluorescence:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University).
Staining:Article Title: Adult-born dentate granule cells promote hippocampal population sparsity
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for immunofluorescence staining: Prox1 anti-rabbit, AngioBio, Catalog# 11-00P; GFP anti-chicken, Aves Labs, Catalog# GFP-1020; NeuN guinea pig, Synaptic Systems Catalog# 266 004; Cy3 donkey anti-rabbit, Jackson ImmunoResearch Catalog# 711-165-152; Cy3 donkey anti-guinea pig, Jackson ImmunoResearch Catalog# 706-165-148; goat antichicken 488, Thermo Fisher Scientific, Catalog# A-11039. .. Validation These antibodies have been validated for immunostaining by the company and/or studies cited on company's website: anti-Prox1 (AngioBio, #11-00P): http://www.angiobio.com/new/product.php?pid=2 anti-GFP (Aves Labs, #GFP-1020): https://www.aveslabs.com/products/anti-green-fluorescent-protein-antibody-gfp anti-NeuN (Synaptic Systems, #266 004): https://sysy.com/product/266004 Cy3 donkey anti-rabbit (Jackson ImmunoResearch, #711-165-152): https://www.jacksonimmuno.com/catalog/products/711-165-152 Cy3 donkey anti-guinea pig (Jackson ImmunoResearch, #706-165-148): https://www.jacksonimmuno.com/catalog/ products/706-165-148 Alexa 488 goat anti-chicken (Thermo Fisher Scientific, #A-11039): https://www.thermofisher.com/antibody/product/Goat-antiChicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 re research | rep o rtin g su m m ary A p ril 2 0 2 0 Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic Nestin-cre mice (The Jackson Laboratories; B6.Cg-Tg(Nes-cre)Kln/J, stock number 003771, RRID: IMSR_JAX:003771; maintained on a C57BL/6J background), Grm2Cre (MMRRC; Tg(Grm2-cre)MR90Gsat/Mmucd; stock # 034611-UCD, RRID:MMRRC_034611-UCD; maintained on a C57BL/6J background) or cFos-tTA (The Scripps Research Institute and maintained at Tufts University).
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